DAB stain, mouse stomach, 40X.
This particular staining protocol had diaminobenzidine, hemotoxylin, and McGill's modified EA solution as stains. The diaminobenzidine, from what I understand, was supposed to color neutrophils and monocytes varying shades of grey-black intracellularly. Light blue is supposed to correspond to basophils. Either I've discovered some new property of basophils in which they invade and form sheets in the connective tissues and basement membrane of the stomach (which'd be even weirder considering that the system in question skews to TH1/TH17-driven inflammation), or I've screwed up the staining protocol somehow. The latter is much more likely, and it also looks like I left the slides in the hemotoxylin for way too long.
I just wish I could remember where I'd put the Coplin jars, because this time around I was staining out of Petri dishes (Toaster does not recommend putting xylenes in a Petri dish, unless it is a fancy glass Petri dish).
Posts
