06 January, 2010

Worms in My Data

This is real flow cytometry data I collected. The terraced blotches represent different populations of cells as differentiated by staining their surfaces with fluorescent antibodies, as such the axes represent 2 different fluorophores' distinct staining intensity. Non-floaty blobs like the sandworm blob are common because primary cell populations are always somewhat heterogeneous and as such will rarely stain in perfectly discrete pots. I couldn't look at this plot and not see a sandworm, so I made it better.